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. 2020 Jan 30;217(4):e20182091. doi: 10.1084/jem.20182091

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© 2020 Tang et al.

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Figure S4. — Identification of E3 ubiquitin ligases that bind to NLRP3. Related to Fig. 5. (A) Silver staining of GST or GST-NLRP3–binding proteins eluted from lysates of B6 BMDMs primed with LPS and stimulated with ATP for 5 min. (B) LC-MS/MS analysis of GST-NLRP3–binding E3 ubiquitin ligases eluted from lysates of B6 BMDMs primed with LPS and stimulated with ATP, captured, and trypsin digested. The top six hits were selected from three independent experiments.