Figure S2.
Cross-reactive mutant MHC-I neoantigen-specific T cells. (A and B) CD8 T cells (5 × 105 CD4-depleted splenocytes/well) from control (Ctr., n = 1) or immunized (Im., n = 3) mice with mutant SLPs were isolated following the in vivo protocol shown in Fig. 1 B and in vitro cultured with mutant or WT counterpart SLPs (A; 25 µg/ml) or SSPs (B; 0–2.5 µg/ml) to assess cross-reactivity through IFN-gamma release by ELISpot assay. Representative data of IFN-gamma spots (mean ± SEM) from MC-38 mutations (M#), CT-26 (C#), and EMT-6 (E#) are shown. Each experiment was independently repeated twice.