Table 2. Mutation efficiency of pCas/pTarget system.

Expt. no.	Targeting genome locus of sgRNA	Host cell	Plasmid pTarget	Length of homology arm (left, right) (bp)	Number of picked colonies /recombinant colonies	Mutation efficiency (%)
1	elt I	E. coli O141:K85	pTarget-Δelt I	137,132	50/2	4.00
2	estB	E. coli O141:K85	pTarget-ΔestB	167,226	68/1	1.40
3	estB	E. coli O141:K85 Δelt I	pTarget-ΔestB	167,226	85/15	17.64
4	faeG	E. coli O141:K85	pTarget-ΔfaeG	203,202	21/5	23.80
5	faeG	E. coli O141:K85 Δelt IΔestB	pTarget-ΔfaeG	203,202	50/2	4.00
6	estB	E. coli O141:K85	pTarget-ΔestB::estA	167,226	99/14	14.14
7	estA	E. coli O142	pTarget-ΔestA	112,101	115/9	7.80
8	elt II c1	E. coli DN1502	pTarget-Δelt II c1(400)	404,465	75/51	68
9	elt II c1	E. coli DN1502	pTarget-Δelt II c1p::faeG	309,428	40/36	90
10	faeG	E. coli DN1502 Δelt II c1::faeG	pTarget-ΔfaeG:: elt II c1193	309,428	20/20	100
11	elt II c1	E. coli DN1502	pTarget-Δelt II c1(300)	304,331	42/54	77.78
12	elt II c1	E. coli DN1502	pTarget-Δelt II c1(200)	235,202	26/43	60.47
13	elt II c1	E. coli DN1502	pTarget-Δelt II c1(100)	124,103	1/60	1.67
14	elt II c1	E. coli DN1502	pTarget-Δelt II c1(50)	49,49	0/19	0

Notes.

Experiments 1, elt I was deleted from E. coli O141:K85. Experiments 2, estB was deleted from E. coli O141:K85. Experiments 3, estB was deleted from O141:K85 Δelt I. Experiments 4, faeG was deleted from E. coli O141:K85. Experiments 5, faeG was deleted from E. coli O141:K85 Δelt IΔestB. Experiments 6, estA was replaced in estB loci in E. coli O141:K85. Experiments 7, estA was deleted from E. coli O142. Experiments 8, elt II c1 was deleted from DN1502 with 400 bp homology arm. Experiments 9–10, the codon of the 193rd amino acid (leucine) of elt II c1 was changed from CTG to CTC. Experiments 11–14, elt II c1 was deleted from DN1502 with different length of homology arms.