Fig. 2.

A general experimental and computational workflow for improving NGS data quality of virus quasispecies studies. For the comparative studies or clinical samples, we start from different samples (1,2,i,…,n). One has to first go through different experimental steps, such as sample preparation, library preparation, library quality control, sample indexing, library pooling and sequencing. Then, computational steps are followed, such as data cleaning, consensus-based error correction, variant calling and annotation.