REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
APC Goat Anti-mIgG	BioLegend	Cat# 405308; RRID: AB_315011
Anti-His/APC	Miltenyi Biotec	Cat# 130-119-820; RRID: AB_2751870
Anti-Flag/FITC	Miltenyi Biotec	Cat# 130-119-683; RRID: AB_2811325
Goat anti-mIgG/Alexa Fluor 594	ZSGB-BIO	ZF-0513
DAPI	Beyotime	Cat# C1002
Murine anti-Flag antibody	Sigma-Aldrich	F1804; RRID: AB_262044
Fixation/Permeabilization Solution Kit	BD Biosciences	Cat# 554714
Bacterial and Virus Strains
Escherichia coli (E. coli) strain DH5α	TIANGEN	Cat# CB101-02
MAX Efficiency DH10Bac Competent E. coli	Invitrogen	Cat# 10361-012
Chemicals, Peptides, and Recombinant proteins
PEI	Alfa	A04043896-1g
Recombinant SARS-CoV-2-S1 protein fused with mFc, spike residues 20-685, accession number: EPI_ISL_402119	This paper	N/A
Recombinant SARS-CoV-2-NTD protein fused with mFc, spike residues 20-286, accession number: EPI_ISL_402119	This paper	N/A
Recombinant SARS-CoV-2-CTD protein fused with mFc, spike residues 319-541, accession number: EPI_ISL_402119	This paper	N/A
Recombinant MERS-RBD protein fused with mFc, spike residues 367-606, accession number: JX869050	This paper	N/A
Recombinant SARS-RBD protein fused with mFc, spike residues 306-527, accession number: NC_004718	This paper	N/A
Recombinant hCD26 protein, residues 39−766, accession number: NP_001926	This paper	N/A
Recombinant hACE2 protein, residues 19−615, accession number: BAJ21180	This paper	N/A
Recombinant hAPN protein, residues 66−967, accession number: NP_001141	This paper	N/A
Critical Commercial Assays
HisTrap HP 5 mL column	GE Healthcare	Cat# 17524802
HiLoad 16/600 Superdex 200 pg	GE Healthcare	Cat# 28989335
Series S Sensor Chip CM5	GE Healthcare	Cat# 29149603
HiTrap Protein G HP	GE Healthcare	Cat# 17040503
Mouse Antibody Capture Kit	GE Healthcare	Cat# BR-1008-38
Deposited Data
Crystal structure of SARS-CoV-2-CTD/hACE2	This paper	PDB: 6LZG; NMDC: NMDCN0000001
Experimental Models: Cell Lines
Sf9 Cells, SFM Adapted	Invitrogen	Cat# 11496015
Hi5 cells	Invitrogen	Cat# B85502
Huh7 cells	Institute of Basic Medical Sciences CAMS	3111C0001CCC000679
HEK293T cells	ATCC	ATCC CRL-3216
Recombinant DNA
pEGFP-N1	MiaoLingPlasmid	Cat# P0133
pEGFP-N1-hACE2, accession number: BAJ21180	This paper	N/A
pEGFP-C1	MiaoLingPlasmid	Cat# P0134
pEGFP-C1-hCD26, accession number: NP_001926	This paper	N/A
pEGFP-C1-hAPN, accession number: NP_001141	This paper	N/A
pFastbac1	Invitrogen	10360014
pFastbac-hCD26-His, residues 39−766, accession number: NP_001926	This paper	N/A
pFastbac-hACE2-His, residues 19−615, accession number: BAJ21180	This paper	N/A
pFastbac-hAPN-His, residues 66−967, accession number: NP_001141	This paper	N/A
pCAGGS	MiaoLingPlasmid	Cat# P0165
pCAGGS-SARS-CoV-2 S-Flag, accession number: EPI_ISL_402119	This paper	N/A
pCAGGS-MERS-CoV-S-Flag, accession number: JX869050	This paper	N/A
pCAGGS-SARS-CoV-S-Flag, accession number: NC_004718	This paper	N/A
pCAGGS-SARS-CoV-2-S1-mFc, residues 20-685, accession number: EPI_ISL_402119	This paper	N/A
pCAGGS-SARS-CoV-2-NTD-mFc, residues 20-286, accession number: EPI_ISL_402119	This paper	N/A
pCAGGS-SARS-CoV-2-CTD-mFc, residues 319-541, accession number: EPI_ISL_402119	This paper	N/A
pCAGGS-MERS-RBD-mFc, residues 367-606, accession number: JX869050	This paper	N/A
pCAGGS-SARS-RBD-mFc, residues 306-527, accession number: NC_004718	This paper	N/A
Software and Algorithms
PyMOL software	Molecular Graphics System, Version 1.8 Schrödinger	https://pymol.org/2/
MEGA version X	Tamura et al., 2013	https://www.megasoftware.net/
BIAcore® 8K Evaluation software	GE Healthcare	N/A
FlowJo V10	FLOWJO	https://www.flowjo.com/solutions/flowjo/downloads
ESPript 3	Robert and Gouet, 2014	http://espript.ibcp.fr/ESPript/ESPript/
Graphpad Prism 6	GraphPad Software	https://www.graphpad.com/
HKL2000	Otwinowski and Minor, 1997	N/A
Phaser	Read, 2001	N/A
COOT	Emsley and Cowtan, 2004	https://www2.mrc-lmb.cam.ac.uk/personal/peemsley/coot/
Phenix	Adams et al., 2010	http://www.phenix-online.org/
MolProbity	Williams et al., 2018	N/A
SigmaPlot	Systat Software, Inc	https://systatsoftware.com/products/sigmaplot/