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. Author manuscript; available in PMC: 2021 Mar 19.
Published in final edited form as: Cell Chem Biol. 2020 Feb 3;27(3):292–305.e6. doi: 10.1016/j.chembiol.2020.01.008

Figure 1. Identification of inhibitors of ASH activity.

Figure 1.

A. Schema showing that inorganic phosphate (Pi) from the Hsp90-mediated hydrolysis of ATP, complexes with molybdate ((NH4)6Mo7O24) to form phosphomolybdate ((NH4)3PMo12O40) that can then bind to the quinaldine red (QR) dye to form a colored product that quenches the inherent fluorescence of the white microtiter plates. B. Scatterplot of the HTS to identify inhibitors of the ASH activity. The data are shown as a percent inhibition of the ASH activity, where Hsp90+Aha1 is set as 0% inhibition (green triangles) and Aha1 is set as 100% inhibition (red triangles). Chemical structure of SEW84 (C) and SEW77 (D).