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. 2020 Mar 13;25(6):1320. doi: 10.3390/molecules25061320

Figure 2.

Figure 2

EVO arrests the cell cycle and induces apoptosis in PC-3 and DU145 cells. (A) PC-3 (5 × 103 cells/well) and DU145 cells (5 × 103 cells/well) were treated with EVO and HGF t the indicated concentrations and for the indicated times. The cells were digested with RNase A for 1 h, then stained with propidium iodide and subjected to flow cytometric analysis to monitor the cell cycle. (B) PC-3 (5 × 103 cells/well) and DU145 cells (5 × 103 cells/well) were treated with EVO or HGF at the indicated concentrations and for the indicated times. The cells were fixed, stained with TUNEL assay reagent, and then analyzed by flow cytometry. NT and EVO (−)/HGF (−) indicate control samples treated with medium containing 0.1% DMSO.