Table 2.
LoD of RT-LAMP Assays Targeting SARS-CoV-2
| RNA copies | SSIV, 10 U/rxn |
SSIV, 20 U/rxn |
||||||
|---|---|---|---|---|---|---|---|---|
| Nsp3_1-61 |
Nsp3_2-24 |
Nsp3_1-61 |
Nsp3_2-24 |
|||||
| Tt_av | RPR | Tt_av | RPR | Tt_av | RPR | Tt_av | RPR | |
| 1000 | 13.11 | 3:3 | 9.31 | 3:3 | 10.7 | 3:3 | 7.53 | 3:3 |
| 500 | 13.47 | 3:3 | 8.16 | 2:3 | 10.63 | 3:3 | 7.59 | 3:3 |
| 200 | 13.65 | 2:3 | 11.19 | 2:3 | 11.34 | 2:3 | 7.78 | 3:3 |
| 100 | 14.62 | 3:3 | — | — | 12.26 | 2:3 | 8.47 | 2:3 |
| 50 | — | — | — | — | — | — | — | — |
| 20 | 13.88 | 1:3 | — | — | — | — | — | — |
| 5 | — | — | — | — | — | — | — | — |
| NTC | — | — | — | — | — | — | — | — |
—, no amplification observed; LoD, limit of detection; RPR, ratio of positive replicates; RT-LAMP, reverse transcription loop-mediated isothermal amplification; rxn, reaction; SARS-CoV-2, severe acute respiratory syndrome coronavirus 2; SSIV, SuperScript IV Reverse Transcriptase; Tt_av, average threshold time in minutes; NTC, no template control.