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. 2019 Sep 8;16(5):946–955. doi: 10.1080/15548627.2019.1664144

Figure 5.

Figure 5.

Interaction between HLA-DR and citrullinated VIM (cVIM) in synovial fibroblasts (SFs). RASFs were treated with IFNG for 72 h followed by serum-free starvation in the last 2 h and their lysates were co-immunoprecipitated with anti-HLA-DR antibodies and blotted with anti-HLA-DR or -cVIM antibodies (A). Proximity ligation assay to demonstrate the formation of HLA-DR-cVIM complexes. RA and OA SFs were left untreated, starved in serum-free medium, or treated with IFNG in the presence or absence of 3-MA. Figures are representative images of 3 independent experiments using cells from 3 different patients (C). Scale bar: 50 μm. Representative cell of Civ (B). Scale bar: 100 μm. Quantification of HLA-DR-cVIM PLA complexes in RASFs (D). Values are presented as mean PLA signal per cell ± S.D. * = P < 0.05. Comparison the quantification of HLA-DR-cVIM PLA complexes in RASFs and OASFs (E). Values are presented as mean PLA signal per cell ± S.D * = P < 0.05.