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. 2020 Feb 14;48(7):3922–3934. doi: 10.1093/nar/gkaa092

Figure 2.

Figure 2.

Determining the Alr1240 regions responsible for the interaction between AnaRne and Alr1240. (A) Schematic diagram of the domains in E. coli RNase R (EcRnr), E. coli RNase II (EcRnb) and Alr1240. All these proteins contain two cold shock domains (CSD), one exoribonuclease domain (RNB) and one S1 RNA-binding domain (S1). EcRnr also contains one helix-turn-helix domain (HTH) at the N-terminus. Based on the domain architecture, three regions of Alr1240 (Alr1240-CSD, Alr1240-RNB and Alr1240-S1) were chosen for the interaction assays. (B) Far-western blot assay assessing the interactions between AnaRneN/AnaRneC and different regions of Alr1240. The experiment procedure was same as that in Figure 1B. (C) Bacterial adenylate cyclase two-hybrid assay investigating the interactions between AnaRneN and different regions of Alr1240. The experiment procedure was same as that in Figure 1C.