Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Mar 6;48(7):3708–3721. doi: 10.1093/nar/gkaa151

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

PMC Copyright notice

Figure 2. — Ligation failure of pol β 8-oxodGTP insertion products. (A) Lanes 1 and 8 are the negative enzyme controls of the 1-nt gapped DNA substrates A^coupled and C^coupled, respectively. Lanes 2–7 and 9–14 show the insertion coupled to ligation products from 8-oxodGTP:A and 8-oxodGTP:C, respectively, obtained at the time points 0.5, 1, 3, 5, 8 and 10 min. (B) Illustrations of the 1-nt gapped DNA substrate with template A or C and the insertion, ligation and ligation failure reaction products obtained in the coupled reaction including 8-oxodGTP. (C andD) The graphs show the time-dependent changes in the ligation (yellow for 8-oxodGTP:A and green for 8-oxodGTP:C) and ligation failure (red) products. The data are presented as the averages from three independent experiments ± SDs.