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. 2020 Mar 6;48(7):3708–3721. doi: 10.1093/nar/gkaa151

Figure 6.

Figure 6.

Ligation efficiency of the repair intermediates with 3′-preinserted mismatches by DNA ligase I and DNA ligase III/XRCC1 complex. (A) Lanes 1, 8 and 15 are the negative enzyme controls of the nicked DNA substrates with 3′-dG:G, 3′-dT:G and 3′-dA:G, respectively, and lanes 2–7, 9–14 and 16–21 show the reaction products obtained with DNA ligase I at the time points 0.5, 1, 3, 5, 8 and 10 min. (C) Illustrations of the nicked DNA substrate with template A, T, G or C (N) and the ligation and ligation failure reaction products obtained in the reaction including 3′-preinserted mismatches (dNmismatch). (B) Lanes 1, 8 and 15 are the negative enzyme controls of the nicked DNA substrates with 3′-dC:C, 3′-dT:C and 3′-dA:C, respectively, and lanes 2–7, 9–14 and 16–21 show the reaction products obtained with DNA ligase III/XRCC1 complex at the time points 0.5, 1, 3, 5, 8 and 10 min.