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. 2020 Feb 27;48(7):e42. doi: 10.1093/nar/gkaa099

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 8. — Impacts of host (human) genomic DNA in human haploid genome equivalents (HHGE) on specific and non-specific amplification. Plots of T_m as a function TTP using Bst 2.0 at (A) 0 HHGE per μl; (B) 0.01 HHGE per μL, (C) 1 HHGE per μl, (D) 100 HHGE per μl and (E) 5000 HHGE per μl; and using Bst 3.0 at (F) 0 HHGE per μl, (G) 0.01 HHGE per μl, (H) 1 HHGE per μl, (I) 100 HHGE per μl (J) 5000 HHGE per μl in the presence of template (blue) and NTC (red). N = 3 for all conditions, except Bst 3.0 at 0 and 100 HHGE per μl in the presence of template, where N = 6.