Table 1.
Advantages and Limitations of TEAA-free and other commonly used ON-MS methods
| TEAA-based ON-MS | TEAA free low-resolution MS | TEAA free high-resolution MS | |
|---|---|---|---|
| Analysis of native RNA | |||
| • Known sequence | yes | yes | yes |
| • Unknown sequence | yes | no | yes |
| Analysis of synthetic ONs | yes | yes | yes |
| Analysis of ONs longer 10 nts | no | no | no |
| Compatibility with small compound analyses | no | yes | yes |
| LC and MS contamination | yes | no | no |
| 2D analysis (57) | yes | no | no |
| Sensitivity | Lower compared to nucleoside MS | Lower compared to nucleoside MS | Lower compared to nucleoside MS |
| Quantitative accuracy and precision | (39–43) | Not fully determined | Not fully determined |