Table 1.

KD values of Tag interacting with ssDNA 57mer

	SV40 Large T antigen binding to
	ssDNA w/o MgAc & ATP	ssDNA with MgAc (5 mM)	ssDNA with ATP (1 mM)	ssDNA with MgAc (5 mM) and ATP (1 mM)
Conditions	KD (nM)	KD (nM)	KD (nM)	KD (nM)
DSF
GAPDH	30±10	10±1	100±20	1,500±1100
Site 2 (DSF)	40±7	50±10	100±30	200±60
EMSA
GAPDH (1)	80±40	30±10	160±40	40±10
GAPDH (2)	120±20	50±10	170±30	140±70
Site 2 (1)	40±5	20±6	80±30	70±8
Site 2 (2)	60±9	60±4	170±50	65±10
Site 2 (1)*	70±10*	40±10*	160±60*	130±60*

(1) EMSA complex 1; (2) EMSA complex 2; * determined after crosslinking of products.