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KD values Tag binding to dsDNA
| SV40 large T antigen binding to | ||
|---|---|---|
| dsDNA w/o MgAc/ATP | dsDNA with MgAc (5 mM) and ATP (1 mM) | |
| Conditions | K D (nM) | K D (nM) |
| DSF | ||
| GAPDH | 5800±4000 | 600±340 |
| Site 2 | 120±110 | 160±40 |
| EMSA | ||
| GAPDH (1) | >>1000 | >>1000 |
| Site 2 (1) | 100±50 | 220±130 |
(1) EMSA Complex 1.
