Figure 1.

Identification of airway epithelial cell selective open chromatin across the CFTR locus. (A) Open chromatin mapping by Omni- ATAC-seq on Caco2 (bright blue), Calu3 (teal), 16HBE14o- (Dark blue), DNase-seq on HBE cells (light blue) and H3K27Ac occupancy by ChIP-seq on Calu3 cells (purple) and 16HBE14o- cells (gray). The location of ASZ1, CFTR and CTTNBP2 are shown at the top of the figure. Major DHS identified at the CFTR locus in all cells that express the gene and other cell-selective DHS of interest are marked below the gene track in black. This manuscript uses legacy nomenclature for the CFTR gene to be consistent with our earlier work. The −35 and −44 kb region is marked by a bar below the tracks. (B) Enlarged image of the −35 and −44 kb DHS regions.