Table 1.
Selected crystallographic data and refinement statistics
| Unit Cell [abc, Å; αβγ, °] | a = 65.7, b = 65.8, c = 82.0 |
| α = 105.9, β= 92.3, γ = 119.9 | |
| Space Group | P1 |
| Wavelength [Å] | 1.000 |
| X-ray Source | APS SER-CAT 22-BM |
| Resolution [Å] (Highest shell) | 50.0–3.00 (3.05–3.00) |
| No. of Reflections [unique] | 18 997 |
| Completeness [%] | 83.8 (53.1) |
| Rsyma [%] | 10.6 (35.0) |
| I/(σI) | 10.3 (2.4) |
| Redundancy | 1.8 (1.5) |
| Refinement Summary | |
| Resolution [Å] (Highest shell) | 25.69–3.00 (3.05–3.00) |
| Hfq protomers/DNA base pairs/Unit Cell | 12/40 |
| No. of non-solvent atoms/Unit Cell | 8055 |
| No. of waters/Unit Cell | 59 |
| Rworkb [%] | 21.1 (23.4) |
| Rfreec [%] | 26.0 (27.9) |
| Average B factor [Å2] | |
| Protein | 48.6 |
| DNA | 81.6 |
| RMS deviations | |
| Bonds [Å] | 0.006 |
| Angles [o] | 1.13 |
| Ramachandran analysis [%] | |
| Favored, allowed, disallowed | 94.9, 5.1, 0.0 |
| PDB ID: code | 5UK7 |
a R sym = Σ|I- 〈I〉|/ Σ|I|, where I is the observed intensity and is the average intensity of several symmetry-related observations.
b R work = Σ||Fo|-|Fc||/ Σ|Fo, where Fo and Fc are the observed and calculated structure factors, respectively.
c R free = Σ||Fo|-|Fc||/ Σ|Fo for 5% of the data not used at any stage of the structural refinement.