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. 2020 Mar 5;48(7):3987–3997. doi: 10.1093/nar/gkaa149

Table 2.

Ec Hfq binding to select DNA sequences

Hfq Kd (nM)
150 mM NaCl binding buffer
Full length, WT Hfq-xtal* 175.0 ± 4.5
Full length, dA16 >3000
Hfq(2-69)-xtal 94.0 ± 8.0
Hfq(2-69)-dA16 >3000
75 mM NaCl binding buffer
Hfq(2-69)-xtal 4.9 ± 2.4
Hfq(2-69)-3base A-tract DNA 19.4 ± 6.0
Hfq(2-69)-RacA DNA 16.2 ± 3.7
Hfq(2-69:N13A)-xtal 6.0 ± 2.3
Hfq(2-69:R16A)-xtal 34.1 ± 7.0
Hfq(2-69:R16A/R17A)-xtal 66.8 ± 10.6
Hfq(2-69:Q41E)-xtal 47.1 ± 4.7
Hfq(2-69:Q41A)-xtal 299.6 ± 33.6

*xtal indicates the DNA sequence used for crystallization.