Figure 4.

Examples for RT-signatures of m¹A, m¹G and m²₂G by RT from revolver oligo analyses and the expected structural impairment of the Watson–Crick base-pairing. Graphs from the revolver oligo with a neighboring A, 3′ adjacent (+1 position) to the modified site at position 9, are shown. Sites with error rates of more than 10% are highlighted with yellow arrows. Colored bars indicate the nature of the reads. The mismatch rate is depicted as black cross and the arrest rate as red line. The modified site is shown at position 9 in the middle of the considered sequence. In general, arrest rate percentages refer to the reads covering the 3′ adjacent position of m¹A/m¹G/m²₂G (+1 position). Mismatch rate percentages refer to the reads covering the modified position; Note that statements on average values stated in the text may differ from these individual signatures.