Figure 3.
Binding of HapX and the CBC on the promoter region of the selected 33 HapX-regulated genes. In vivo binding of VenusHapX and HapCGFP on the 5′-upstream region of the 33 HapX-regulated genes are shown. In cases where no A. fumigatus gene designation was assigned, we used the gene name of the respective S. cerevisiae ortholog. Tracks for the ChIP-seq (ChIP), their input DNA control (Input), and the binding specificity controls for HapX (NLS-Venus ChIP) are visualized in the UCSC genome browser together with annotated gene models and their transcript, which are expressed in RPMI-1640 culture conditions. RNA-seq data were taken from (49). Direction of the target gene and the proximal genes are shown in red arrows and blue arrows, respectively. The positions of the nucleotide motif matched with the consensus binding sequence for the CBC identified from our ChIP-seq analysis (5′-CCAATVR-3′) are also shown.