Figure 4.

(A) MEME analysis of 36 A. fumigatus CBC:HapX sites identified an A/T-rich trinucleotide (5′-RWT-3′) located 12 bp downstream of the CBC binding site. (B) The sequence logo of Yap1-like (TTAN) and Gcn4-like half-sites (TGAN) found in the 3′-submotifs of 32 CBC:HapX sites was generated by WebLogo (54). The distance between the CCAAT box and HapX 5′-T(T/G)AN-3′ half-site consensus motifs shows a remarkable variation (C), whereas their DNA strand orientation tends to be distance specific with preferred positions at 14(+) and 20(−) nt downstream of the CBC consensus motif (D).