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. 2020 Feb 4;48(7):3869–3887. doi: 10.1093/nar/gkaa067

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 3. — The group B homology domain does not confer sequence selective RNA binding. (A) Multiple sequence alignment of mouse SoxB1 group done with ClustalX 2.0.9 (http://www.clustal.org/download/2.0.9/). The DNA-binding HMG domains are framed in red. The RNA-binding motif (RBM) of Sox2 and its N-terminal part corresponding to the group B homology domain are boxed in green or blue, respectively. (B) Recombinant Sox1 resolved by SDS-PAGE and visualized by Coomassie staining. M: protein size markers in kDa. (C, D) Comparison of the binding of Sox1 to 12th-24 RNA and its mutants. Representative EMSAs are shown in (C) and the fractional RNA binding is plotted in (D). The concentrations of Sox1 are as indicated and complexes are labelled to the right. Data are represented as mean ± SD (n = 2).