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. 2020 Mar 30;16(3):e1008412. doi: 10.1371/journal.ppat.1008412

Fig 4. Detection of HeV protein products in infected ferret lung tissues using LC-MS/MS.

Fig 4

(A) The HeV genome consists of six genes (3’-N, P, M, F, G, L-5’). A polar transcription gradient is formed where 3’ genes are transcribed more frequently than genes further downstream. These genes give rise to the six structural HeV proteins N, P, M, F, G and L. RNA editing of the P gene can also yield V and W protein and transcription of an alternative reading frame of the P gene produces C protein. (B) Using LC-MS/MS, five HeV structural proteins were detected. The number of peptides (#Peps) and percentage of protein coverage (%Cov) are listed. For each observed HeV protein, a schematic diagram representing the peptide regions of protein detected by LC-MS/MS is shown. The position and size of each peptide are to scale. The arrow represents the point at which the amino acid sequence of the P, V and W proteins differ downstream (position 405).