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. 2020 Apr 9;15(4):e0231267. doi: 10.1371/journal.pone.0231267

Fig 4. Mitochondrial respiration in MLEC isolated from male and female mice.

Fig 4

Mitochondrial function in male and female MLEC and HPAEC was measured using XF24 Analyzer (Seahorse Biosciences, North Billerica, MA). Oligomycin (1μM), FCCP (1μM), and Rotenone and Antimycin A (1μM each) were added at the indicated time points (A, F). Basal mitochondrial respiration for MLEC (B) and HPAEC (G), maximal respiratory capacity for MLEC (C) and HPAEC (H), ATP production for MLEC (D) and HPAEC (I), and Proton leak for MLEC (E) and HPAEC (J) were found to be significantly lower in female MLEC and HPAEC compared to male. Values are means ± SEM. For MLEC, one out of two independent experiments is shown. N = 3 repetitions from 5–7 mice isolated for each sex. For HPAEC, one out of two independent experiments is shown. N = 10 for male and N = 9 repetitions for female donor. *p < 0.05 < 0.05 versus male MLEC. Statistical analysis was performed using the unpaired t-test.