KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Chemicals, Peptides, and Recombinant Proteins | ||
| Proteinase K | Sigma-Aldrich | Cat#P2308; CAS: 39450-01-6 |
| Ammonium sulfate | Sigma-Aldrich | Cat#A4418; CAS: 7783-20-2 |
| Deposited Data | ||
| Atomic coordinates, proteinase K crystal structure used for molecular replacement | (Hattne et al., 2018) | PDB: 6CL7 |
| Atomic coordinates and density map of proteinase K (Table 1, #2) | This paper | PDB: 6PKJ; EMDB: EMD-20356 |
| Atomic coordinates and density map of proteinase K (Table 1, #5) | This paper | PDB: 6PKK; EMDB: EMD-20357 |
| Atomic coordinates and density map of proteinase K (Table 1, #7) | This paper | PDB: 6PKL; EMDB: EMD-20358 |
| Atomic coordinates and density map of proteinase K (Table 1, #8) | This paper | PDB: 6PKM; EMDB: EMD-20359 |
| Atomic coordinates and density map of proteinase K (Table 1, #9) | This paper | PDB: 6PKN; EMDB: EMD-20360 |
| Atomic coordinates and density map of proteinase K (Table 1, #12) | This paper | PDB: 6PKO; EMDB: EMD-20361 |
| Atomic coordinates and density map of proteinase K (Table 1, #10) | This paper | PDB: 6PKP; EMDB: EMD-20362 |
| Atomic coordinates and density map of proteinase K (Table 1, #11) | This paper | PDB: 6PKQ; EMDB: EMD-20363 |
| Atomic coordinates and density map of proteinase K (Table 1, #13) | This paper | PDB: 6PKR; EMDB: EMD-20364 |
| Atomic coordinates and density map of proteinase K (Table 2, low-dose no platinum/LD) | This paper | PDB: 6PKS; EMDB: EMD-20365 |
| Atomic coordinates and density map of proteinase K (Table 2, low-dose with platinum/LDPT) | This paper | PDB: 6PKT; EMDB: EMD-20366 |
| Software and Algorithms | ||
| TVIPS tools | (Hattne et al., 2015) | N/A |
| XDS | (Kabsch, 2010b) | RRID:SCR_015652 |
| XSCALE | (Kabsch, 2010a) | RRID:SCR_015652 |
| Phaser | (McCoy et al., 2007) | RRID:SCR_014219 |
| Phenix.refine | (Afonine et al., 2012) | RRID:SCR_014224 |
| PyMol | (Schrodinger LLC, 2014) | RRID:SCR_000305 |
| Other | ||
| TEM grids | Quantifoil | N/A |
| easiGlow glow discharge cleaning system | PELCO | N/A |
| Vitrobot Mark IV plunge-freezer | Thermo Fisher | N/A |
| FEI Autoloader | Thermo Fischer | N/A |
| FEI Talos Arctica | Thermo Fischer | N/A |
| FEI CetaD | Thermo Fischer | N/A |
| FEI Versa FIB/SEM | Thermo Fischer | N/A |