Fig 4. Dopaminergic antagonists inhibit CsAd chemotaxis to cholic acid.

One CsAd in each trough was incubated in 1× Locke’s solution containing LE-300 (A), SKF-83566 (B), raclopride (C), CS-49612 (D), or BTCP (E), for 10 min at the starting line, and stimulated with 100 mM CA. The migration distance of the CsAds was measured every 10 min for 2 h. Each chemical, at 100 mM, was also tested as a stimulant to determine its own chemotactic effect (blue triangle up). Asterisks indicate * p < 0.05, ** p < 0.01 and *** p < 0.001 compared to the CA-only control (red square) at 120 min.