Figure 3.

Protein–ligand interaction (PLI) detection. A) Biotin (black) and AZA (magenta) were titrated with a constant concentration of SA (400 nM) and CA (200 nM), respectively, using a 1 nM Eu-probe. The monitored EC₅₀ values with both ligands are in stoichiometry with their target proteins. B) T_m values for SA (red, 400 nM) and CA (blue, 200 nM) were monitored with and without the saturating ligand concentration of biotin (black 10 μM) and AZA (magenta, 5 μM), respectively. Data represent mean ± SD (n = 3).