Table 1. Protein Properties and the Obtained Melting Temperatures.

				Tm
				without ligand		with ligand
	Mw (kDa)	pI	ng per well	measd	lit.	measd	lit.
CA	30	6.6	48	67.9 ± 0.9	71.8a	69.8 ± 0.7	73.2a
SA	53	5	170	75.7 ± 0.1	75.5b	>90	112b
MDH	70	6.1–6.4	51	44.6 ± 0.5	46c	ND	ND
IgG1	150	6.5–9	60	70.7 ± 0.2	71d	ND	ND

^a2 μM CA(II) in 100 mM Tris buffer (pH 7.5) with 0.36 μM AZA using absorbance 280 measurement.

^b66 μM SA in 100 mM phosphate buffer (pH 7.4) with 132 μM biotin using DSC.

^c0.7 μM MDH in 20 mM sodium phosphate buffer (pH 7.0) using CD spectroscopy.

^d130 μM IgG in 10 mM phosphate buffer (pH 8.1) using DSC.