Skip to main content
. 2020 Mar 23;25(6):1441. doi: 10.3390/molecules25061441

Figure 2.

Figure 2

Temporal changes [Ca2+]i in GT1-7 cells after exposure to amyloidogenic proteins. (A) Temporal changes in [Ca2+]i shown as pseudocolor images. AβP(1–40) (10 µM) was applied to GT1–7 cells, and temporal changes in fluorescence intensities corresponding to changes in [Ca2+]i were analyzed by multi-site fluorometry using fura-2. (a): 1 min before exposure to AβP(1–40); (b): 20 s after exposure; (c): 5 min after exposure. (B) Typical time course of [Ca2+]i changes before and after exposure to each amyloidogenic protein (10 µM). (a) AβP(1–40); (b) AβP(40–1); (c) AβP(1–42); (d) D-AβP(1–40); (e) PrP106–126; (f) scramble PrP106–126; (g) human amylin (hIAPP); (h) rat amylin; (i) NAC; (j) magainin 2. The arrow indicates the time of peptide addition (modified from Ref. No. 15 with permission).