Figure 5.
eGFP expression in HSC2 cells after pDNA administration by sonoporation with NBs. (A, C) Low power magnified fluorescent microscopic image stained with hoechst 33342 taken 24 h after sonoporation. (B, D) High power magnified image stained with hoechst 33342 and anti-GFP antibody. (A, B) sonicated sample with pDNA encoding eGFP and L- NBs. (C, D) negative control without pDNA, NBs and sonication. Scale bars: 1,000 μm (A, C), 50μm (B, D).