Figure 3. Hyperosmotic stress-induced adaptive gene expression occurs independent of cell type and osmolyte used.

(A) Control and shc-Rel MEFs were treated with 5% or 10% DSS. RNA was isolated, and mRNA transcripts analyzed via RT-qPCR. (B) Control and shc-Rel MEFs were treated with 5% or 10% DSS. Cytoplasmic membrane fractions were isolated, and protein levels analyzed via western blot. (C) Control and shc-Rel MEFs were treated with 5% or 10% DSS. Total lysates were analyzed via western blot. (D) RAW macrophages were treated with 5% or 10% DSS for the indicated durations. RNA was isolated, and transcripts were analyzed via qPCR. (E) Primary tracheal epithelial cells cultured from human patients were treated with the indicated concentration of NaCl. RNA was isolated, and transcripts were analyzed via qPCR.