Table 1. Twenty-nine experiments (until April 2019) from 15 different papers reporting amnestic effects of post-reactivation midazolam (MDZ) administration for contextual fear conditioning in adult rats (in chronological order based on publication date).
Only studies that adopted protocols similar to the ones used in the current article were selected (including contextual fear conditioning and post-reactivation systemic MDZ injection in rats). The effect size (Cohen’s d) for the influence of amnestic treatment on % freezing during the test session was estimated from reported graphs (MDZ versus vehicle). Details of the intervention, such as duration of the reactivation session and drug dose, are indicated as well. Franzen et al. (2019) found no amnestic effect during initial testing after MDZ injection following a longer (5-min) re-exposure session, but the effect was only apparent after a ‘reinstatement’ procedure. The numbers in the second column refer to the lab of the corresponding author:
- IFEC-CONICET, Departamento de Farmacología, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba, Argentina.
- School of Psychology, University of New South Wales, Sydney, Australia.
- Department of Pharmacology, Federal University of Santa Catarina, Florianópolis, Santa Catarina, Brazil.
- Laboratorio de Psicología Experimental, Facultad de Psicología, Universidad Nacional de Córdoba, Córdoba, Argentina.
- Department of Neuropsychopharmacology, National Institute of Mental Health, National Center of Neurology and Psychiatry, Tokyo, Japan.
| Publication | Lab | Exp. | Fig. | Duration reactivation session | MDZ dose | Ntotal | Effect size (Cohen’s d) |
|---|---|---|---|---|---|---|---|
| Bustos et al. (2006) | I | 1B | 2B | 90 s | 1 mg/kg | 16 | 2.23 |
| Bustos et al. (2006) | I | 2A | 3B | 90 s | 1 mg/kg | 18 | 2.75 |
| Bustos et al. (2006) | I | 3B | 7B | 90 s | 1 mg/kg | 16 | 4.87 |
| Zhang & Cranney (2008) | II | 1 | 1 | 90 s | 2 mg/kg | 18 | .96 |
| Zhang & Cranney (2008) | II | 3 | 3 | 90 s | 2 mg/kg | 16 | 1.83 |
| Bustos et al. (2009) | I | NA | 1C | 3 min | 1.5 mg/kg | 14 | 3.87 |
| Bustos et al. (2009) | I | NA | 1D | 5 min | 1.5 mg/kg | 20 | 3.08 |
| Bustos et al. (2009) | I | NA | 3B | 3 min | 1.5 mg/kg | 14 | 3.35 |
| Bustos et al. (2010) | I | 1 | 1B | 3 min | 1.5 mg/kg | 14 | 2.62 |
| Bustos et al. (2010) | I | 1 | 1B | 3 min | 3 mg/kg | 14 | 5.74 |
| Bustos et al. (2010) | I | 1 | 1C | 5 min | 1.5 mg/kg | 18 | 2.50 |
| Bustos et al. (2010) | I | 1 | 1C | 5 min | 3 mg/kg | 18 | 2.47 |
| Stern et al. (2012) | III | 1 | 1A | 3 min | 1,5 mg/kg | 14-24 | 1.49 |
| Piñeyro et al. (2013) | IV | 1 | 1C | 4 min | 3 mg/kg | 12 | 3.82 |
| Piñeyro et al. (2013) | IV | 1 | 1C | 5 min | 3 mg/kg | 12 | 3.21 |
| Alfei et al. (2015) | IV | 5 | 5A | 2 min | 3 mg/kg | 18 | 1.80 |
| Alfei et al. (2015) | IV | 6 | 6B | 5 min | 3 mg/kg | 14 | 2.77 |
| Ortiz et al. (2015) | I | 1 | 1C | 3 min | 3 mg/kg | 19 | 3.37 |
| Ortiz et al. (2015) | I | 1 | 1E | 5 min | 3 mg/kg | 12 | 4.46 |
| Ortiz et al. (2015) | I | 2 | 3C | 5 min | 3 mg/kg | 16 | 4.33 |
| Ferrer Monti et al. (2016) | IV | 1 | 1C | 4 min | 3 mg/kg | 14 | 2.98 |
| Espejo et al. (2016) | I | 1 | 1 | 5 min | 3 mg/kg | 15 | 4.05 |
| Espejo et al. (2016) | I | 2 | 2 | 5 min | 3 mg/kg | 22 | 3.03 |
| Saitoh et al. (2017) | V | NA | 2B | 3 min | 1 mg/kg | 24 | .88 |
| Ferrer Monti et al. (2017) | IV | 2 | 2B | 2 min | 3 mg/kg | 12 | 3.88 |
| Espejo et al. (2017) | I | 1 | 1 | 5 min | 3 mg/kg | 16 | 3.49 |
| Espejo et al. (2017) | I | 3 | 3 | 5 min | 3 mg/kg | 18 | 3.91 |
| Akagi et al. (2018) | V | NA | 2C | 3 min | 1 mg/kg | 30 | .87 |
| Franzen et al. (2019) | III | NA | 1B | 2 min | 3 mg/kg | 17 | 2.45 |