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. Author manuscript; available in PMC: 2020 Sep 30.
Published in final edited form as: Nat Cell Biol. 2020 Mar 30;22(4):487–497. doi: 10.1038/s41556-020-0489-9

Fig. 5. Endothelial-specific sites bound by TAL1 in vitro highlight known haemato-endothelial enhancers.

Fig. 5

a, UMAP as in Fig. 1b with a box around the allantoic-haemato-endothelial cells (left). Zoomed in UMAP (right) shows and describes the cells found in this landscape only. b, UMAP of allantoic-haemato-endothelial cells (n=3,284 cells) showing the enrichment scores (colour gradient from grey=0 to dark blue=1) for TAL1 ChIP-seq peaks obtained from in vitro-derived haemangioblasts, haemogenic endothelium and haematopoietic progenitors from32. ChIP-seq peaks were taken from http://codex.stemcells.cam.ac.uk/. c, Genome browser tracks showing the Erg (top) and the Fli1 (bottom) loci. Black arrowheads indicate the Erg +85kb (top) and the Fli1 -15kb (bottom) enhancers. Tracks correspond to the snATAC-seq profiles of the erythroid, endothelium and allantois cell types after cell pooling, the TAL1 ChIP-seq for haemogenic endothelial cells (“TAL1 ChIP-seq HE”, grey) and H3K27ac ChIP-seq for haemogenic endothelial cells from32 (“H3K27ac HE”, gold). Haemogenic endothelial TAL1 and H3K27ac ChIP-seq tracks were obtained from http://codex.stemcells.cam.ac.uk/.