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. Author manuscript; available in PMC: 2020 Sep 30.
Published in final edited form as: Nat Cell Biol. 2020 Mar 30;22(4):487–497. doi: 10.1038/s41556-020-0489-9

Fig. 6. Flt1 +67kb and Maml3 +360kb are two endothelial-specific enhancers.

Fig. 6

Left: Genome browser tracks showing the Flt1 (a) and the Maml3 (b) loci. Black arrowheads indicate the Flt1 +67kb (top) and the Maml3 +360kb (bottom) enhancers. Tracks correspond to the snATAC-seq profiles of the erythroid, endothelium and allantois cell types after cell pooling, the TAL1 ChIP-seq for haemogenic endothelial cells (“TAL1 ChIP-seq HE”, grey) and H3K27ac ChIP-seq for haemogenic endothelial cells from32 (“H3K27ac HE”, gold). Haemogenic endothelial TAL1 and H3K27ac ChIP-seq tracks were obtained from http://codex.stemcells.cam.ac.uk/. Right: E11.5 transgenic mouse embryos generated by pronuclear injection of lacZ reporter constructs into fertilized zygotes, followed by transfer into pseudopregnant recipient females. LacZ activity was visualised with X-Gal staining. Both the Flt1 +67kb (top) and Maml3 +360kb (bottom) enhancers show specific staining in the vasculature (black arrowheads as example). This was observed in 8/15 and 2/5 stained embryos for Flt1 +67kb and Maml3 +360kb, respectively. See Supplementary Table 9 for numbers of transgenic embryos analysed.