Extended Data Fig. 9. Identification of neurotransmitters expressed in some FBNs/FB2Ns.

Neurotransmitter expression detected in neuron somata using antibody labelling. We identified GAL4 lines that drive gene expression in some of the FBN or FB2N neurons and used them to express GFP in these neurons. We stained central nervous systems with antibodies against GFP and either ChAT (choline acetyltransferase), GABA (gamma aminobutyric acid) or GLUT (vesicular glutamate transporter).Each row shows from left to right: the name of the individual neuron, anti-GFP (green), anti-ChAT (magenta), and both antibody stainings combined; anti-GFP (green), anti-GABA (magenta), and both antibody stainings combined; anti-GFP (green) and anti-GLUT (magenta), and both antibody stainings combined. Whether a cell is cholinergic, GABAergic or glutamatergic is listed at the beginning of each row under the neuron name. Images show confocal maximum intensity projections of specific neuronal cell bodies. At least two replicates were obtained per genotype. Scale bars: 5μm.