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. 2020 Feb 21;32(4):967–983. doi: 10.1105/tpc.19.00772

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© 2020 American Society of Plant Biologists. All rights reserved.

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Figure 5. — Dosage-Dependent HY5 Activity Controls Plant Size in Arabidopsis and Tomato.

(A) Arabidopsis plants of the indicated genotypes growing in short days (8 h of light/16 h of dark) for 42 d. Plants are as follows: 35S:FLASH-HY5-SRDX (HY5-SRDX), 35S:FLASH-HY5ox (HY5ox), 35S:FLASH-HY5ΔN77ox (HY5ΔN77ox), 35S:FLASH-HY5-VP16 (HY5-VP16), and 35S:FLASH-HY5ΔN77-VP16 (HY5ΔN77-VP16). Bar = 3 cm.

(B) HY5-FLASH protein levels from 7-d-old seedlings of the genotypes and growth conditions shown in (A), detected with an anti-FLAG antibody against FLAG-HY5 and FLAG-HY5ΔN77.

(C) Three-week-old tomato plants of the wild type, 35S:DOF-AtHY5ox (OX), 35S:DOF-AtHY5-VP16 (VP16), and 35S:DOF-AtHY5-SRDX (SRDX). DOF, HA-YFP-HA. Bars = 1.5 cm.

(D) Eight-week-old tomato plants. Bar = 5 cm.

(E) Mature tomato plants.