Photosynthetic Inhibitors Modulate eIF2α Phosphorylation in the Light.
(A) Schematic of the light regimen consisting of a long-day photoperiod followed by 24 h dark acclimation and the start time of light treatment (80 µE m−2s−1) and sampling (T = 0).
(B) Time course of eIF2α phosphorylation in 14-d-old wild-type Ler-0 seedlings after lights-on. Thirty minutes prior to light exposure, seedlings were sprayed with either DMSO (mock), 8 µM DCMU, or 16 µM DBMIB.
(C) Schematic of the light regimen for (D).
(D) eIF2α phosphorylation starting at ZT2 (T = 0) either left untreated (LD) or treated with water up to 120 min (mock) or treated with 20 µM MV. For details, see legend to Figure 1.