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Disruption of NRPD1 in Capsella Impairs 24-nucleotide siRNA Formation and RdDM.
(A) Deleted genomic region in Capsella NRPD1 at 1634 to 2108 bp (genomic sequence). Target 1 (T1) and target 2 (T2) sequences of CRISPR/Cas9 are indicated. AA, amino acids; CDS, coding sequence.
(B) Profile of TE-derived siRNAs in Capsella wild-type (wt) and nrpd1 leaves. RPM, reads per million mapped reads.
(C) DNA methylation levels at TEs in Capsella wild-type and nrpd1 leaves.
