Figure 1.
Reduced Seed Set in ketch1/+ Was Due to Female Gametophytic Defect.
(A) to (F) A representative silique from self-fertilized wild type (A) or ketch1-2/+ (B), from ketch1-2/+ pistil pollinated with wild-type pollen (C) or wild-type pistil pollinated with ketch1-2/+ pollen (D) or from two complementation lines, i.e., ProUBQ10:KETCH1-YFP;ketch1-2 (E) and (F).
(G) to (J) Optical sections (G) and (I) or whole-mount clearing (H) and (J) of wild-type (G) and (H) or ketch1-2 (I) and (J) ovules pollinated with wild-type pollen at 24 HAP. Arrows in (G) point at initiating peripheral endosperm; the arrowhead in (G) points at the nucleus of the zygote; the arrow in (I) points at the single nucleus in the embryo sac. Region highlighted in lilac is an elongated zygote in wild type (H) or the single nucleus in the abnormal embryo sac of ketch1-2 (J).
(K) Seed set. Results shown are means ± SD (n = 50 to 100). Means with different letters indicate significant difference (one-way ANOVA, Tukey’s multiple comparison test, P < 0.05). Bars = 2 mm for (A) to (F); 10 µm for (G) to (J).