Figure 3.

KETCH1 Loss-of-Function Compromised PMI during Pollen Development.

(A) Representative semithin transverse sections of developing anthers at stage 9, stage 10, stage 11, or stage 12 from wild type or ketch1-2/+. Arrowheads point at aborted microspores.

(B) to (E) Representative TEM sections of ketch1-2/+ anthers at stage 11, showing a wild-type-looking microspore (B) and defective microspores at early (C), middle (D), or late (E) stages. Arrowhead in (C) points at invaginated PM. N, Nucleus; GN, generative nucleus; VN, vegetative nucleus. Bars = 20 µm for (A); 5 µm for (B) to (E).