Figure 5.
KETCH1 Loss of Function Compromised Pollen Tube Guidance.
(A) and (B) SEMs of a wild-type (A) or a ketch1-2/+ ovule (B) pollinated with wild-type pollen at 12 HAP. Pollen tubes are shown in lilac.
(C) and (D) Histochemical GUS staining of a wild-type (C) or a ketch1-2/+ pistil (D) pollinated with ProLAT52:GUS pollen at 12 HAP. Two overlapping high-magnification images were taken for one pistil. The images were then overlaid with Photoshop (Adobe) to show the whole pistil. Arrowheads point at the ovules not targeted by a pollen tube.
(E) and (F) Aniline blue staining of a wild-type (E) or a ketch1-2/+ pistil (F) pollinated with wild-type pollen at 48 HAP. Arrowheads in (F) point at unfertilized ovules. Results shown at the bottom of (C) to (F) are means ± SD (n = 20). The ketch1-2/+ mutant is significantly different from wild type (t test, P < 0.05). Bars = 20 µm for (A) and (B); 100 µm for (C) and (D); 200 µm for (E) and (F).