Figure 7.

KETCH1 Interacts with Several RPs.

(A) BiFC assays showing the interaction between KETCH1 and several RPs, including RPL23A, RPS7, RPS3A, RPL27aC, and RPL27aB. U1-70k-mCherry was used to label the nucleus and to mark transformed cells. Positive interactions were highlighted as insets. BiFC-positive signals are shown in green while U1-70k-mCherry is in magenta. From top to bottom, the YFP channel, the RFP channel, merges of the YFP, RFP, and transmission channels. For each combination, 30 pavement cells from five tobacco infiltrated leaves were examined with the same results.

(B) In vitro pull-down showing that KETCH1 interacts with RPL27aC. Experiments were performed three times with similar results. Bars = 20 µm.