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. 2020 Jan 27;32(4):1049–1062. doi: 10.1105/tpc.19.00617

Figure 6.

Figure 6.

JAZ Proteins Affect the Transcriptional Function of RHD6.

(A) Schematic of the effectors and reporters used in the transient transactivation assays. Pro35S, CaMV 35S promoter; ProRSL4, RSL4 promoter.

(B) Transient dual-luciferase reporter assays. JAZ4 and JAZ8, not JAZ4-N (amino acids 1 to 176) and JAZ8-N (amino acids 1 to 74), interfere with RHD6 to modulate the expression of RSL4. Error bars indicate SD from three biological replicates using different batches of plants.

(C) Transient dual-luciferase reporter assays. 100 μM MeJA attenuates the inhibition of JAZ4 on RHD6 transcription. Error bars indicate SD from three independent biological replicates using different batches of plants.

(D) Transient transcriptional activity assays. Activation of the RSL4 promoter by RHD6 is decreased in the coi1-2 and coi1-16. Error bars indicate SD from five independent biological replicates using different batches of plants. Bars with different letters are significantly different from each other (P < 0.05). Data were analyzed by ANOVA.