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. 2019 Nov 16;47(22):11889–11905. doi: 10.1093/nar/gkz1059

Table 1.

Description of transcript ends originating from the psbB operon

End number Genome position Notes
TSS, +TAP 5′ ends
1 72 200 PpsbB -171, described in (57). Seen in barley
2 72 409 Internal to psbB. Distal promoter for psbT?
3 74 393 PpsbH -92, described in (60)
4 76 153 Internal to petB exon 2. Distal promoter for petD?
5 76 375–76 376 Upstream petD
6 76 391 Internal to petD intron
7 76 780 Internal to petD intron
8 75 482 Antisense to petB intron. Distal promoter for psbN ?
Processed 5′ ends, −TAP 5′ends
1 72 320 psbB -51 mature end, described in (57)
2 73 211 Internal to psbB. Highest peak from a region with multiple 5′ends. Degradation intermediate?
3 73 658 Internal to psbB. Highest peak from a region with multiple 5′ends. Degradation intermediate?
4 74 418 psbT-psbH intergenic region, psbH -67 mature end, described as a precise endoribonuclease cleavage in (60). See also 3′ end #7
5 74 441 psbT-psbH intergenic region, psbH -44 mature end. Main psbH 5′ end, processing depends on HCF107, described in (58,59)
6 74 794 psbH-petB intergenic region, petB -47 mature end. Processing depends on HCF152, described in (61,62). See 3′ end #9
7 74 847 first nucleotide of petB intron. Sign of a hydrolytic splicing?
8 76 627 internal to petD intron. Degradation intermediates?
9 76 679 internal to petD intron. Degradation intermediates?
10 76 760 internal to petD intron. Degradation intermediates?
11 76 830 internal to petD intron. Degradation intermediates?
12 76 863 internal to petD intron. Highest peak from a region with multiple 5′ ends. Degradation intermediates?
3′ ends
1 72 601 Internal to psbB. Degradation intermediate?
2 72 786 Internal to psbB. Degradation intermediate?
3 73 371 Internal to psbB. Degradation intermediate? Downstream of numerous 5′ends, see 5′end #2, maybe the signature of an endo-ribonuclease cleavage?
4 73 838 Internal to psbB. Degradation intermediate? Downstream of numerous 5′ends, see 5′end #3, maybe the signature of an endo-ribonuclease cleavage?
5 74 082 First nucleotide of psbT, might be the 3′ end of a cDNA identified in (30)
6 74 242 3′ end of psbT, psbT +60, defined by a stem loop that also defines the 3′ end of the antisense psbN transcript, see 3′ end #17. Described in (30)
7 74 405 psbT-psbH intergenic region, 3′ end of psbT, psbT +223, described as a precise endo-ribonuclease cleavage in (60). See also 5′ end #4
8 74 687 Internal to psbH, 20 nt upstream of the stop codon. Degradation intermediate?
9 74 814 psbH-petB intergenic region, psbH +109 mature end. Processing depends on HCF152, described in (61,62). See 5′ end #6
10 76 358 petB-petD intergenic region, petB +67 mature end. Processing depends on CRP1, described in (63)
11 76 543 internal to petD intron. Degradation intermediates?
12 77 014 internal to petD intron. Degradation intermediates? 5 nt downstream of a smRNA footprint.
13 77 047 internal to petD intron. Degradation intermediates?
14 77 765 3′ end of petD, petD +94, defined by a stem loop that also defines the 3′end of the antisense rpoA transcript, see 3′ end #16. Processing requires mTERF6, described in (64)
15 77 892 3′ end of petD, petD +221.
16 77 716 3′ end of rpoA, rpoA +185, defined by a stem loop that also defines the 3′end of the antisense petD transcript, see 3′ end #14. Processing requires mTERF6, described by (64)
17 74 211 3′ end of psbN, psbN +39, defined by a stem loop that also defines the 3′ end of the antisense psbT transcript, see 3′ end #6. Described in (30)