Table 1.
Description of transcript ends originating from the psbB operon
End number | Genome position | Notes |
---|---|---|
TSS, +TAP 5′ ends | ||
1 | 72 200 | PpsbB -171, described in (57). Seen in barley |
2 | 72 409 | Internal to psbB. Distal promoter for psbT? |
3 | 74 393 | PpsbH -92, described in (60) |
4 | 76 153 | Internal to petB exon 2. Distal promoter for petD? |
5 | 76 375–76 376 | Upstream petD |
6 | 76 391 | Internal to petD intron |
7 | 76 780 | Internal to petD intron |
8 | 75 482 | Antisense to petB intron. Distal promoter for psbN ? |
Processed 5′ ends, −TAP 5′ends | ||
1 | 72 320 | psbB -51 mature end, described in (57) |
2 | 73 211 | Internal to psbB. Highest peak from a region with multiple 5′ends. Degradation intermediate? |
3 | 73 658 | Internal to psbB. Highest peak from a region with multiple 5′ends. Degradation intermediate? |
4 | 74 418 | psbT-psbH intergenic region, psbH -67 mature end, described as a precise endoribonuclease cleavage in (60). See also 3′ end #7 |
5 | 74 441 | psbT-psbH intergenic region, psbH -44 mature end. Main psbH 5′ end, processing depends on HCF107, described in (58,59) |
6 | 74 794 | psbH-petB intergenic region, petB -47 mature end. Processing depends on HCF152, described in (61,62). See 3′ end #9 |
7 | 74 847 | first nucleotide of petB intron. Sign of a hydrolytic splicing? |
8 | 76 627 | internal to petD intron. Degradation intermediates? |
9 | 76 679 | internal to petD intron. Degradation intermediates? |
10 | 76 760 | internal to petD intron. Degradation intermediates? |
11 | 76 830 | internal to petD intron. Degradation intermediates? |
12 | 76 863 | internal to petD intron. Highest peak from a region with multiple 5′ ends. Degradation intermediates? |
3′ ends | ||
1 | 72 601 | Internal to psbB. Degradation intermediate? |
2 | 72 786 | Internal to psbB. Degradation intermediate? |
3 | 73 371 | Internal to psbB. Degradation intermediate? Downstream of numerous 5′ends, see 5′end #2, maybe the signature of an endo-ribonuclease cleavage? |
4 | 73 838 | Internal to psbB. Degradation intermediate? Downstream of numerous 5′ends, see 5′end #3, maybe the signature of an endo-ribonuclease cleavage? |
5 | 74 082 | First nucleotide of psbT, might be the 3′ end of a cDNA identified in (30) |
6 | 74 242 | 3′ end of psbT, psbT +60, defined by a stem loop that also defines the 3′ end of the antisense psbN transcript, see 3′ end #17. Described in (30) |
7 | 74 405 | psbT-psbH intergenic region, 3′ end of psbT, psbT +223, described as a precise endo-ribonuclease cleavage in (60). See also 5′ end #4 |
8 | 74 687 | Internal to psbH, 20 nt upstream of the stop codon. Degradation intermediate? |
9 | 74 814 | psbH-petB intergenic region, psbH +109 mature end. Processing depends on HCF152, described in (61,62). See 5′ end #6 |
10 | 76 358 | petB-petD intergenic region, petB +67 mature end. Processing depends on CRP1, described in (63) |
11 | 76 543 | internal to petD intron. Degradation intermediates? |
12 | 77 014 | internal to petD intron. Degradation intermediates? 5 nt downstream of a smRNA footprint. |
13 | 77 047 | internal to petD intron. Degradation intermediates? |
14 | 77 765 | 3′ end of petD, petD +94, defined by a stem loop that also defines the 3′end of the antisense rpoA transcript, see 3′ end #16. Processing requires mTERF6, described in (64) |
15 | 77 892 | 3′ end of petD, petD +221. |
16 | 77 716 | 3′ end of rpoA, rpoA +185, defined by a stem loop that also defines the 3′end of the antisense petD transcript, see 3′ end #14. Processing requires mTERF6, described by (64) |
17 | 74 211 | 3′ end of psbN, psbN +39, defined by a stem loop that also defines the 3′ end of the antisense psbT transcript, see 3′ end #6. Described in (30) |