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Pif1, but not mtSSBs, aids Mip1 in DNA synthesis by through G-quadruplexes. (A) Cartoon showing the different polymerases and single-stranded DNA binding proteins found in the nucleus and mitochondria in yeast. (B) Schematic of the primer extension reaction scheme. (C) Quantification of the fraction of full-length product generated by Mip1 alone (black) or in the presence of either ScRim1 (red) or Pif1 (blue) or both (green), with cMyc (left) and hTelo (right) in the template strand.
