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. 2019 Jul 22;47(16):8581–8594. doi: 10.1093/nar/gkz606

Figure 2.

Figure 2.

The acidic tip peptide, P15, binds with specificity to four SIPs. The results of ITC titrations of P15 peptide into four SIPs at 25°C, (A) RecO (B) PriA (C) χ in buffer BTP (pH 8.0, 50 mM NaCl) and (D) PriC in buffer BTP (pH 8.0, 10 mM NaCl). A titration of P15 with PriC at 50 mM NaCl showed no binding. Upper panels show the raw titration data, plotted as the heat signal (microcalories per second) versus time (minutes), obtained for 22 injections (12 μl each) of P15 (50 μM) into a SIP solution (1–2 μM). Lower panels show the integrated heat responses per injection, normalized to the moles of injected P15, after subtraction of the heats of dilution obtained from the blank titration of P15 into buffer (empty squares). The smooth curves represent the best fit of the data to an n-independent and identical site model. Binding parameters from the fits are indicated in each panel as well as in Table 1 and Supplementary Table S1 (N = stoichiometry, K = association equilibrium binding constant, ΔH = binding enthalpy).