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. 2019 Jul 22;47(16):8581–8594. doi: 10.1093/nar/gkz606

Figure 6.

Figure 6.

RecO binding to the linker deletion variants of SSB tetramer–(dT)70 complexes. Results of ITC experiments for RecO binding to complexes of (dT)70 bound to the SSB tetramers: (A)-wtSSB, (B)-SSBΔ151-166, (C)-SSBΔ130-166 and (D)-SSBΔ120-166. SSB–(dT)70 complexes (9–10 μM) were titrated into RecO (1 μM) in buffer BTP (pH 7.0, 200 mM NaCl) at 25°C. The smooth curves are simulations of Equations (1) and (2) using the best fit parameters determined from each titration. The ΔH for RecO binding per C-terminus is indicated on each panel. The binding parameters are shown in Table 3.