Figure 6.

Sequence variants of indels at the junctions. (A) 4 bp 5′-protruding ends were generated by BsmBI digestion prior to microinjection. Sequence variants of the transgene–transgene head-to-tail junction region (Var1-Var93) are aligned below the original sequence. In most of the junctions, end processing removed only 5′-overhangs. (B) We calculated junction variant frequency for all detected mutations. The diagram shows the distribution of the top three variants (Var1, 2, 3) and remaining variants (‘all other’) in analyzed embryos.