Table 3.

¹H NMR chemical shifts δ_H (ppm) for CpG DNA duplex 7a (top) and its m⁵C-methylated counterpart 7b (bottom) observed at 25°C in D₂O with WATERGATE suppression. The representative 2D NOESY spectrum is given in Supplementary Figure S3. For calculation of sugar-base proton distances, see Supplementary Data

^aObserved at 10°C in 9:1 buffer/D₂O solvent mix. The buffer used was 10 mM sodium phosphate buffer (pH 7.4) containing 150 mM NaCl and 20 mM MgCl₂.

^bChemical shift for 5-methyl group on m⁵C.

^cNot observed even at 4°C.